12 October 2009

 

Screening Soybean Varieties for Resistance to Iron Chlorosis, 2009

 

R. Jay Goos and Brian Johnson, Department of Soil Science, NDSU, Fargo, ND 58108-6050

e-mail:  rj.goos@ndsu.edu

 

How the experiments were conducted

 

Field studies were set out at five locations in 2009, to measure the resistance of over 300 soybean varieties to iron chlorosis.  Two sites in Richland County were lost to ponding that occurred after the plots had emerged.  The remaining three sites were located near Ayr, Hunter, and Leonard, ND.  Soil tests are given in Table 1.  The sites ranged in pH from 8.0 to 8.4, salinity (EC) from 0.4 to 0.9 mmho/cm, and CaCO3 contents ranging from about 3 to 15 %.  Each plot consisted of a hill, where eight seeds were planted in a hill, thinned soon after emergence to three plants.  Prior research has shown that the results given by three plants per hill are about the same as given by soybeans planted in 30-inch rows at a normal plant population. The experimental design was a randomized complete block with four replications at each site.   There were separate trials for Roundup Ready and conventional varieties at each site.

 

Visual ratings were made on a 1-5 scale, with 1=representing no chlorosis and 5= the most severe chlorosis.   Ratings were taken at the 2-3 trifoliolate and 5-6 trifoliolate stages. Thus, each three-site average shown in the data tables (Tables 2-5) is an average of 24 observations (3 sites x 4 replicates x 2 ratings per plot). 

 

In the conventional variety trial, six ÒstandardÓ varieties were entered, to help in the interpretation of the results.  The varieties, listed in expected order from the most resistant to most susceptible, were Iowa State  ISU A11 >  Traill  > Council  > Glacier >Mycogen 5072 = Stine 0480. In the Roundup-Ready trial, three varieties representing the range of chlorosis resistance in commercial varieties were included as standards.  All standards were entered twice, to provide information on the reproducibility of our ratings.

 

The year 2009 was a "bad chlorosis year."  The fall and winter of 2008-2009 were exceptionally wet, and resulted in historic flooding in the southern Red River Valley.  The early part of the growing season also turned wetter than normal, especially in Richland County.  We lost two of our trials, but reliable data were obtained at all the other three locations.  About 130 varieties were entered in both 2008 and 2009.  In general, the results for 2009 were similar to the results obtained in 2008 (Figure 1). 

 

 

The "Chlorosis Report Card"

 

Last year, we introduced the concept of the chlorosis "report card."  We received many favorable comments about this system.  We still report the numerical results (2.2, 3.1, etc.), but a disadvantage of this method is that chlorosis scores, like yields, go up and down from year to year.  Thus, a score of "2.5" in one year may not mean the same thing as a rating of "2.5" in another year.  Thus, we are experimenting with a second way of interpreting the data, "The Chlorosis Report Card."

 

The Chlorosis Report Card gives a variety a letter grade, from A to D-, representing how a variety did, compared to the other offerings in the marketplace.  The range in the scores of commercial varieties is divided into eleven categories (A, A-, B+, B, B-, C+, C, C-, D+, D, D-), and the varieties placed into these eleven categories.   In other words, the most resistant commercial variety defined the top end of the "A" range, and the most susceptible commercial variety defined the bottom of the "D-" range.  We did not give a variety an "A+" grade, because we have never seen a variety immune to chlorosis.  We did not give out any "F" grades either, as no commercial variety has proven to be as susceptible as the two most susceptible varieties identified (T203 and Pride B216).

 

For chlorosis-prone land, a good interpretation of this system would be:

1.  Flee the D's!  These varieties are not adapted for chlorosis-prone land.

2.  You can do better than a C!  Many varieties exist with better chlorosis resistance.  

3.  A grade of B or B- is a good grade, especially for fields that tend to have slight to moderate chlorosis for a couple weeks and recover, or where chlorosis is present some years and not others.  By going with a B, B-, or stronger, you are selecting from the top 25% of the varieties on the market with regards to chlorosis resistance.

4.  For the fields with significant chlorosis problems, consider a variety with a grade of A, A-, or B+, consistent with your other objectives, such as maturity.  This represents about the top 5-10% of the varieties with regards to chlorosis resistance.

5.  Taking on new land?  Be careful.  If a farmer is buying or renting new land, the soil test shows calcium carbonate in the topsoil, pH values above 7.6, and the farmer is not sure of the chlorosis history of the field, the farmer should probably be conservative, and go with a variety with higher levels of resistance.

6. Chlorosis ratings are not perfect.  Chlorosis is a variable problem within a field, and chlorosis ratings are affected by experimental error. Ratings do vary from year to year (Figure 1).  Consistency of performance is important for selecting varieties for fields with severe chlorosis problems.  A variety with a proven performance across more than one year's trials is a safer bet for chlorosis-prone land than a new variety with a limited track record.

7.  No chlorosis?  Then these charts aren't for you!  Not every production area, not every field, has chlorosis.  Chlorosis is generally only seen on poorly-drained fields, or fields with lime in the topsoil.  The letter grades listed here deal only with chlorosis.  It is often observed that a variety may have a chlorosis grade of C or D but be an excellent choice for a field with no chlorosis problems.

 

 

The summary of the chlorosis scores for the Roundup Ready trial, from most to least resistant, is shown in Table 2. The results for the Roundup Ready trial, sorted by company, are shown in Table 3.

 

The results for the conventional trial, sorted by chlorosis score, are shown in Table 4. The results for the conventional trial, sorted by company, are shown in Table 5.

 

 

The authors thank the North Dakota Soybean Council for their support of these trials.