R. Jay Goos and Brian Johnson, Department of Soil Science, NDSU, Fargo, ND 58105
e-mail: rj.goos@ndsu.edu
Field studies were set out at five locations in 2005, to measure the resistance of about 180 soybean varieties to iron chlorosis. The sites were located near Arthur, Ayr, Chaffee, Colfax, and Galesburg, ND. The sites ranged in pH from 7.8 to 8.1, salinity (EC) from 0.4 to 1.9 mmho/cm, and CaCO3 contents ranging from 2 to 11 %. Thirty five seeds were planted in short (5Õ) rows on 30-inch centers. The experimental design was a randomized complete block with four replications at each site.
Visual ratings were made on a 1-5 scale, with 1=representing no chlorosis and 5= the most severe chlorosis. Ratings were taken at the 2-3 and 5-6 trifoliolate stages.
Ten ÒstandardÓ varieties were entered, to help in the interpretation of the results. The varieties, listed in general order from the most resistant to most susceptible, were Iowa State ISU A11 > Seeds 2000 2070RR > Traill = Council > Asgrow 0801 = Peterson PFS 0202RR > Glacier >Mycogen 5072 = Stine 0480 = NuTech 0505RR. The variety ISU A11 represents the highest level of chlorosis resistance available in current breeding lines. All standards were entered twice, to show the degree of variation in our ratings.
The summary of the chlorosis scores, averaged across site and the three ratings, are shown in Table 1. Each chlorosis rating in Table 1 represents the average of 40 observations. In general, very good data were obtained in 2005. Our standard varieties separated themselves out as expected, and reproducibility of our standards was very good.
If a field produces temporary chlorosis, a level of chlorosis from which plants normally recover in a week or two, we generally recommend that varieties have a chlorosis resistance in the range of Asgrow 0801 or better. On fields with more serious chlorosis problems, where yellowing persists, and plants growth is stunted, only varieties with the highest levels of resistance should be planted, consistent with other considerations, such as maturity.
This is the fifth year of this project. We think that it is appropriate, after five years, to look back at the data that has been generated. Our recommendations are based the performance of ÒstandardÓ varieties. We think that it is appropriate, after five years, to look back on the consistency of our data for these standard varieties.
Figure 1 shows the chlorosis scores of our standard varieties, averaged across site and time of rating, for each year. It is important to note that the lines are more-or-less parallel to each other. In other words, we have observed that the strongest varieties are the strongest varieties, and that the weakest varieties are the weakest varieties. There is some minor Òcrossing overÓ in ratings, which is likely due to the experimental error involved with doing chlorosis research.
Figure 1. Average chlorosis scores for our ÒstandardÓ varieties, averaged across site, for the first five years of this project. North Dakota.

This report is also available online at:
This research was sponsored by the
North Dakota Soybean Council.
Table 1. Average chlorosis scores, across two ratings and five
locations, North Dakota, 2005.
|
Company |
Variety |
Average |
|
Standards |
A11 |
1.4 |
|
Standards |
A11 |
1.5 |
|
Asgrow |
AG 00603 |
2.0 |
|
Peterson Farm Seed |
06004 RR |
2.0 |
|
Standards |
S2000 2070RR |
2.1 |
|
Stine |
0056-4 |
2.1 |
|
Dyna-Gro |
30B04 |
2.1 |
|
Ziller |
Exp 75103R |
2.1 |
|
Stauffer |
4004 R |
2.1 |
|
Mycogen |
5B004 RR |
2.1 |
|
Wensman |
W20051 RR |
2.1 |
|
Brushvale |
BS 0535 N |
2.2 |
|
Gold Country |
GCS 426 RR |
2.2 |
|
Legend |
LS 0255 RR |
2.2 |
|
Hyland |
HX 254 |
2.2 |
|
Standards |
S2000 2070RR |
2.2 |
|
Stine |
0063-4 |
2.2 |
|
Univ.-MN |
M98-210060 |
2.2 |
|
Legend |
LS 0036 RR |
2.3 |
|
Stauffer |
4020 R |
2.3 |
|
Stine |
0066-34 |
2.3 |
|
Univ.-MN |
M00-103043 |
2.3 |
|
ProSeed |
RR 50-04 |
2.3 |
|
Croplan |
RT 0043 |
2.3 |
|
Company |
Variety |
Average |
|
Brushvale |
BS 0533 N |
2.3 |
|
Thunder |
2502 RR |
2.4 |
|
Pioneer |
90M01 |
2.4 |
|
Stine |
0260-4 |
2.4 |
|
Hyland |
RR Reliant |
2.4 |
|
Asgrow |
AG 0401 |
2.4 |
|
Gold Country |
GCS 926 RR |
2.4 |
|
Wensman |
W2020 RR |
2.4 |
|
Standards |
Traill |
2.5 |
|
Standards |
Council |
2.5 |
|
ProSeed |
RR 50-06 |
2.5 |
|
Thunder |
2501 RR |
2.5 |
|
Gold Country |
GCS 825 RR |
2.5 |
|
Standards |
Traill |
2.5 |
|
Production Partners |
0099 RR |
2.5 |
|
Legend |
LS 0045 RR |
2.5 |
|
Sabre |
0624 RR |
2.5 |
|
Standards |
Council |
2.5 |
|
Pioneer |
90M60 |
2.6 |
|
Legend |
LS 0093 RR |
2.6 |
|
Asgrow |
AG 0301 |
2.6 |
|
Kaystar |
K-0520 RR |
2.6 |
|
ProSeed |
RR 50-07 |
2.6 |
|
Ziller |
BT 7102R |
2.6 |
|
Company |
Variety |
Average |
|
Mallard |
RR 0212 |
2.6 |
|
Univ.-MN |
M98-211117 |
2.6 |
|
Stine |
0090-64 |
2.6 |
|
Asgrow |
AG 0202 |
2.6 |
|
Midwest Seed Genetics |
GR 0504 |
2.6 |
|
Stine |
0070-64 |
2.7 |
|
Garst |
1226 RR |
2.7 |
|
Thunder |
26009 RR |
2.7 |
|
Hyland |
HX 54001 RR |
2.7 |
|
NuTech |
NT-0090 RR |
2.7 |
|
Mycogen |
X55009 RR |
2.7 |
|
NorthStar |
NS 0620 RR |
2.7 |
|
Legend |
LS 0624 RR |
2.7 |
|
Dyna-Gro |
30M09 |
2.7 |
|
Croplan |
RT 0273 |
2.7 |
|
Wensman |
W20092 RR |
2.7 |
|
Peterson Farm Seed |
06006RR |
2.7 |
|
NuTech |
NT-0121+ RR |
2.7 |
|
Standards |
PFS 0202 RR |
2.7 |
|
ProSeed |
RR 40-71 |
2.7 |
|
NorthStar |
NS 00990RR |
2.7 |
|
NorthStar |
NS 0130 RR |
2.7 |
|
NorthStar |
NS 0911 RR |
2.7 |
|
Croplan |
RT 0669 |
2.7 |
|
Company |
Variety |
Average |
|
ProSeed |
RR 50-00 |
2.7 |
|
Kruger Seed |
K-009+RR |
2.7 |
|
Prairie Brand |
PB-00965 RR |
2.7 |
|
Dairyland |
DST C9-000/RR |
2.8 |
|
Univ.-MN |
M99-230063 |
2.8 |
|
Standards |
PFS 0202 RR |
2.8 |
|
Thunder |
26005 RR |
2.8 |
|
Gold Country |
GCS Sonora |
2.8 |
|
Hyland |
RR Royal |
2.8 |
|
Sabre |
0061 |
2.8 |
|
Standards |
Asgrow 0801 RR |
2.8 |
|
Univ.-MN |
M00-108066 |
2.8 |
|
Sabre |
0092 |
2.8 |
|
Wensman |
W20077 RR |
2.8 |
|
Dairyland |
DSR-0501/RR-STS |
2.8 |
|
ProSeed |
RR 50-10 |
2.8 |
|
Prairie Brand |
PB-0554 RR |
2.8 |
|
NorthStar |
NS 0110 RR |
2.8 |
|
Production Partners |
0444+ RR/STS |
2.8 |
|
Peterson Farm Seed |
EXP 0.8RR |
2.8 |
|
Wensman |
W2064 RR |
2.8 |
|
Standards |
Asgrow 0801 RR |
2.8 |
|
Mallard |
RR 0611 |
2.8 |
|
Mycogen |
X 55081 RR |
2.8 |
|
Company |
Variety |
Average |
|
Garst |
0549 RR |
2.9 |
|
Hyland |
HX 540 |
2.9 |
|
NuTech |
NT-0525+ RR |
2.9 |
|
Sabre |
0883 CNRR |
2.9 |
|
Dyna-Gro |
36N05 |
2.9 |
|
Pioneer |
90M20 |
2.9 |
|
Stauffer |
4051 R |
2.9 |
|
Hyland |
HX 54016 RR |
2.9 |
|
Croplan |
RT 0103 |
2.9 |
|
Garst |
XR05C12 |
2.9 |
|
Peterson Farm Seed |
0606 RR |
2.9 |
|
Ziller |
Exp 45105R |
2.9 |
|
NK Brand |
X 5005 R |
2.9 |
|
Production Partners |
1617+ RR/SCN |
2.9 |
|
Kruger Seed |
K-056 RR |
2.9 |
|
Prairie Brand |
PB-0565 RR |
2.9 |
|
Stauffer |
4005 R |
2.9 |
|
DeKalb |
DKB 09-53 |
2.9 |
|
Seeds 2000 |
2060 RR |
3.0 |
|
Dyna-Gro |
33T06 |
3.0 |
|
NuTech |
NT-0939 + RR |
3.0 |
|
Prairie Brand |
PB-0885 NRR |
3.0 |
|
DeKalb |
DKB 08-51 |
3.0 |
|
NK Brand |
S06-L6 |
3.0 |
|
Company |
Variety |
Average |
|
Standards |
Glacier |
3.0 |
|
Gold Country |
GCS 2605 RR |
3.0 |
|
Thunder |
2604 RR |
3.0 |
|
Mycogen |
5B053 RR |
3.0 |
|
Mycogen |
5B110 RR |
3.0 |
|
Seeds 2000 |
0051 RR |
3.0 |
|
Sabre |
0540 |
3.0 |
|
Standards |
Glacier |
3.0 |
|
Mallard |
RR 0514 |
3.0 |
|
Mallard |
RR 1012 |
3.0 |
|
Prograin |
S6326.22 |
3.0 |
|
Prograin |
S 97215.15 |
3.1 |
|
Wensman |
W2082 RR |
3.1 |
|
Seeds 2000 |
2090 RR |
3.1 |
|
Pioneer |
91M13 |
3.1 |
|
NK Brand |
S02-M9 |
3.1 |
|
Garst |
XR08Y31 |
3.1 |
|
NuTech |
NT-0616+ RR |
3.1 |
|
Garst |
XR 12C5 |
3.1 |
|
Production Partners |
0801 RR/SCN |
3.1 |
|
Midwest Seed Genetics |
GRX-11-01-5 |
3.1 |
|
Ziller |
Exp 44411R |
3.1 |
|
Prograin |
S98216.01 |
3.1 |
|
Kruger Seed |
K-100 RR |
3.1 |
|
Company |
Variety |
Average |
|
Dairyland |
DSR-C800/RR |
3.1 |
|
Midwest Seed Genetics |
GR1633 |
3.1 |
|
Kruger Seed |
K-033 RR |
3.1 |
|
Mycogen |
X 55031 RR |
3.1 |
|
Production Partners |
0102 RR |
3.2 |
|
Seeds 2000 |
2130 RR |
3.2 |
|
Gold Country |
GCS 2603 RR |
3.2 |
|
NK Brand |
S01-T5 |
3.2 |
|
Dyna-Gro |
SX 05203 |
3.2 |
|
Dairyland |
DSR-0401/RR |
3.2 |
|
Ziller |
BT 7065R |
3.2 |
|
Thunder |
2606 RR |
3.2 |
|
Peterson Farm Seed |
0604 RR |
3.2 |
|
NK Brand |
S08-C3 |
3.2 |
|
Midwest Seed Genetics |
GR1111 |
3.3 |
|
Prograin |
S98127.03 |
3.3 |
|
Brushvale |
BS 0520 N |
3.3 |
|
NK Brand |
X50476 RR |
3.3 |
|
NK Brand |
X506 R |
3.3 |
|
Seeds 2000 |
2050 RR |
3.3 |
|
Kaystar |
K-0710 RR |
3.3 |
|
Prairie Brand |
PB-0725 RR |
3.4 |
|
Ziller |
Exp 24104R |
3.4 |
|
NK Brand |
S04-Z9 |
3.4 |
|
Company |
Variety |
Average |
|
Brushvale |
BS 0507 |
3.4 |
|
Midwest Seed Genetics |
GRX-09-02-5 |
3.4 |
|
Mallard |
RR 0914 |
3.5 |
|
Prograin |
S98127.38 |
3.5 |
|
Pioneer |
91M12 |
3.5 |
|
Croplan |
RT 0776 |
3.5 |
|
Kruger Seed |
K-098 RR |
3.6 |
|
Standards |
Mycogen 5072 |
3.6 |
|
Prograin |
S 97126.13 |
3.6 |
|
Standards |
NuTech 0505 RR |
3.6 |
|
NK Brand |
X 508 R |
3.7 |
|
Standards |
Stine 0480 |
3.7 |
|
Standards |
Mycogen 5072 |
3.7 |
|
Standards |
Stine 0480 |
3.7 |
|
NK Brand |
X 511 R |
3.7 |
|
Standards |
NuTech 0505 RR |
3.8 |
|
Brushvale |
BS 0500 N |
3.8 |
|
Midwest Seed Genetics |
GR 0803 |
3.8 |
|
Brushvale |
BS 0565 |
3.8 |
|
Sabre |
0733 CN RR |
3.9 |